Ayperi Aytmırza Kızı

Article | 2020 | Acta Parasitologica65 ( 4 )

Background Dirofilaria immitisandDirofilaria repensare the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan. Purpose The goal of this study was to investigateDirofilariaspecies using PCR and sequencing in dogs from Kyrgyzstan. Method Dirofilariaspp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan. Result The overall prevalence ofDirofilariaspp. was 0.59% (2/337): DNA ofD. immitiswas detected in on . . .e sample and DNA ofD. repensin second positive sample. In second sample, parallel co-infection ofD. repenswithWolbachiawas also found. WhileD. immitissequence showed 98.70-100% similarity with previously reported sequences ofD. immitisfrom dog blood,D. repensshared 100% identity with other sequences ofD. repens. Conclusion These results provided first evidence forDirofilariaspp. in Kyrgyzstan and emphasized the veterinary and medical importance More less

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA | Ayperi MURATBEKOVA

Article | 2021 | Acta Parasitologica66 ( 4 )

Purpose Predatory fungi have been the subject of fundamental studies and their potential as biological control agents against parasitic plant nematodes has been assessed. The aim of the present study was to isolate and identify predatory fungi, performing in vitro and in vivo screening to select highly active strains to control parasitic nematodes. Methods Different nutrient media were used to isolate predatory fungi and determine their morphological and cultural properties. Identification was performed by classical and molecular biology methods. In vitro and in vivo screening was conducted to select highly active strains. Results T . . .welve isolates of Arthrobotrys oligospora (Orbiliomycetes) found in nature were investigated for their predaceous efficacy against garlic stem nematodes (Ditylenchus dipsaci). The effect of temperature and pH on the growth rate and trap formation of representative isolates was determined and isolates were characterized by light microscopy and molecular markers. BLAST was used to sequence the rDNA internal transcribed spacer of A. oligospora isolate KTMU-7. The optimum growth of A. oligospora strains was achieved at 20-25 degrees C on 1-2?orn meal agar (CMA) within the pH range of 5.6-8.6. The factors responsible for the trap formation of these fungal strains were identified. In vitro and in vivo experiments were performed to evaluate the nematicidal activity of local predatory fungal isolates against soil nematodes. Conclusions Preliminary studies proved A. oligospora to be a potentially effective biological control agent, immobilizing 85.7 /- 2.19?f garlic stem nematodes in soil from the rhizosphere of potato plants More less